Journal: BMC Genomics
Article Title: Distinct and overlapping gene regulatory networks in BMP- and HDAC-controlled cell fate determination in the embryonic forebrain
doi: 10.1186/1471-2164-13-298
Figure Lengend Snippet: Western Blot and ELISA Microarray. Proteins were extracted from TSA (10, 25, 50nM) and BMP2 (10 ng/μl) treated neurosphere cultures 6, 12 or 24 h after treatment. Western Blot for pSmad1/5/8, Smad1/5/8, pStat3, Stat3, pGsk3-beta was performed. Beta-Actin was used as control ( A ). Phosphorylated proteins were quantified using an ArrayTube™ (Alere Technologies, Jena, Germany) based sandwich ELISA microarray. Concentration of pGsk3-beta ( B ) and pErk2 ( C ) in relation to total protein content is presented.
Article Snippet: Phosphorylated proteins were quantified using an ArrayTube™ (Alere Technologies, Jena, Germany) based sandwich ELISA microarray, as previously described [ ].
Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Microarray, Sandwich ELISA, Concentration Assay